Cargando…

Highly conserved salt bridge stabilizes a proteinase K subfamily enzyme, Aqualysin I, from Thermus aquaticus YT-1

The proteinase K subfamily enzymes, thermophilic Aqualysin I (AQN) from Thermus aquaticus YT-1 and psychrophilic serine protease (VPR) from Vibrio sp. PA-44, have six and seven salt bridges, respectively. To understand the possible significance of salt bridges in the thermal stability of AQN, we pre...

Descripción completa

Detalles Bibliográficos
Autores principales:	Sakaguchi, Masayoshi, Osaku, Kanae, Maejima, Susumu, Ohno, Nao, Sugahara, Yasusato, Oyama, Fumitaka, Kawakita, Masao
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer 2014
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4131155/ https://www.ncbi.nlm.nih.gov/pubmed/25136511 http://dx.doi.org/10.1186/s13568-014-0059-2

Ejemplares similares

The third restriction–modification system from Thermus aquaticus YT-1: solving the riddle of two TaqII specificities
por: Skowron, Piotr M., et al.
Publicado: (2017)

Establishment of a quantitative PCR system for discriminating chitinase-like proteins: catalytically inactive breast regression protein-39 and Ym1 are constitutive genes in mouse lung
por: Ohno, Misa, et al.
Publicado: (2014)

Chitinase mRNA Levels by Quantitative PCR Using the Single Standard DNA: Acidic Mammalian Chitinase Is a Major Transcript in the Mouse Stomach
por: Ohno, Misa, et al.
Publicado: (2012)

Characterization of a Bacillus thuringiensis chitinase that binds to cellulose and chitin
por: Honda, Shotaro, et al.
Publicado: (2017)

Glucoamylase of Caulobacter crescentus CB15: cloning and expression in Escherichia coli and functional identification
por: Sakaguchi, Masayoshi, et al.
Publicado: (2014)

Quantitative Real-Time PCR Analysis of YKL-40 and Its Comparison with Mammalian Chitinase mRNAs in Normal Human Tissues Using a Single Standard DNA
por: Ohno, Misa, et al.
Publicado: (2015)

The crystal structure of the Thermus aquaticus DnaB helicase monomer
por: Bailey, Scott, et al.
Publicado: (2007)

Complete Genome Sequence of Thermus aquaticus Y51MC23
por: Brumm, Phillip J., et al.
Publicado: (2015)

Quantification of Chitinase mRNA Levels in Human and Mouse Tissues by Real-Time PCR: Species-Specific Expression of Acidic Mammalian Chitinase in Stomach Tissues
por: Ohno, Misa, et al.
Publicado: (2013)

Crystal structure of a Thermus aquaticus diversity-generating retroelement variable protein
por: Handa, Sumit, et al.
Publicado: (2019)

Inhibitory Effect of Bridged Nucleosides on Thermus aquaticus DNA Polymerase and Insight into the Binding Interactions
por: Kim, Sung-Kun, et al.
Publicado: (2016)

Variants of a Thermus aquaticus DNA Polymerase with Increased Selectivity for Applications in Allele- and Methylation-Specific Amplification
por: Drum, Matthias, et al.
Publicado: (2014)

Gastric and intestinal proteases resistance of chicken acidic chitinase nominates chitin-containing organisms for alternative whole edible diets for poultry
por: Tabata, Eri, et al.
Publicado: (2017)

RNA polymerase-promoter interactions determining different stability of the Escherichia coli and Thermus aquaticus transcription initiation complexes
por: Mekler, Vladimir, et al.
Publicado: (2012)

Loss and Gain of Human Acidic Mammalian Chitinase Activity by Nonsynonymous SNPs
por: Okawa, Kazuaki, et al.
Publicado: (2016)

Protein A-Mouse Acidic Mammalian Chitinase-V5-His Expressed in Periplasmic Space of Escherichia coli Possesses Chitinase Functions Comparable to CHO-Expressed Protein
por: Kashimura, Akinori, et al.
Publicado: (2013)

3D structure of Thermus aquaticus single-stranded DNA–binding protein gives insight into the functioning of SSB proteins
por: Fedorov, Roman, et al.
Publicado: (2006)

Functional Properties of the Catalytic Domain of Mouse Acidic Mammalian Chitinase Expressed in Escherichia coli
por: Kashimura, Akinori, et al.
Publicado: (2015)

Protease resistance of porcine acidic mammalian chitinase under gastrointestinal conditions implies that chitin-containing organisms can be sustainable dietary resources
por: Tabata, Eri, et al.
Publicado: (2017)

Incorporation of N7-Platinated Guanines into Thermus Aquaticus (Taq) DNA Polymerase: Atomistic Insights from Molecular Dynamics Simulations
por: De Castro, Federica, et al.
Publicado: (2023)

Acidic Chitinase-Chitin Complex Is Dissociated in a Competitive Manner by Acetic Acid: Purification of Natural Enzyme for Supplementation Purposes
por: Tabata, Eri, et al.
Publicado: (2018)

Chitin digestibility is dependent on feeding behaviors, which determine acidic chitinase mRNA levels in mammalian and poultry stomachs
por: Tabata, Eri, et al.
Publicado: (2018)

High expression of acidic chitinase and chitin digestibility in the stomach of common marmoset (Callithrix jacchus), an insectivorous nonhuman primate
por: Tabata, Eri, et al.
Publicado: (2019)

Identification of Thermus aquaticus DNA polymerase variants with increased mismatch discrimination and reverse transcriptase activity from a smart enzyme mutant library
por: Raghunathan, Govindan, et al.
Publicado: (2019)

Functional Properties of Mouse Chitotriosidase Expressed in the Periplasmic Space of Escherichia coli
por: Kimura, Masahiro, et al.
Publicado: (2016)

Acidic mammalian chitinase is a proteases-resistant glycosidase in mouse digestive system
por: Ohno, Misa, et al.
Publicado: (2016)

Bindel-PCR: a novel and convenient method for identifying CRISPR/Cas9-induced biallelic mutants through modified PCR using Thermus aquaticus DNA polymerase
por: Sakurai, Takayuki, et al.
Publicado: (2019)

Safety evaluation of the food enzyme aqualysin 1 from a genetically modified Bacillus subtilis (strain LMGS 25520)
por: Silano, Vittorio, et al.
Publicado: (2018)

Residues of acidic chitinase cause chitinolytic activity degrading chitosan in porcine pepsin preparations
por: Tabata, Eri, et al.
Publicado: (2019)

Modified ‘one amino acid-one codon’ engineering of high GC content TaqII-coding gene from thermophilic Thermus aquaticus results in radical expression increase
por: Zylicz-Stachula, Agnieszka, et al.
Publicado: (2014)

Microsporidian diversity in the aquatic isopod Asellus aquaticus
por: Grabner, Daniel, et al.
Publicado: (2022)

Crab-Eating Monkey Acidic Chitinase (CHIA) Efficiently Degrades Chitin and Chitosan under Acidic and High-Temperature Conditions
por: Uehara, Maiko, et al.
Publicado: (2022)

Characterization of a New Cyclohexylamine Oxidase From Acinetobacter sp. YT-02
por: Zhou, Hui, et al.
Publicado: (2018)

Antibacterial and antibiofilm potential of Lacticaseibacillus rhamnosus YT and its cell-surface extract
por: Guan, Chengran, et al.
Publicado: (2023)

Developmental Transcriptomic Analysis of the Cave-Dwelling Crustacean, Asellus aquaticus
por: Gross, Joshua B., et al.
Publicado: (2019)

The ER Repeat Protein Yt521-B Localizes to a Novel Subnuclear Compartment
por: Nayler, Oliver, et al.
Publicado: (2000)

shp-2 gene knockout upregulates CAR-driven cytotoxicity of YT NK cells
por: Subrakova, V.G., et al.
Publicado: (2020)

YT521-B homology domain family proteins as N6-methyladenosine readers in tumors
por: Yang, Heng, et al.
Publicado: (2022)

Expression of YT Domain Family Protein in Oral Squamous Cell Carcinoma and Its Correlation with the Prognosis
por: Shi, Rui, et al.
Publicado: (2023)

Into the Thermus Mobilome: Presence, Diversity and Recent Activities of Insertion Sequences Across Thermus spp.
por: Blesa, Alba, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_qjpvo59muqfd5s5sv2fgv1sau6