Single chromosome transcriptional profiling reveals chromosome-level regulation of gene expression

Here we report iceFISH, a multiplex imaging method for measuring gene expression and chromosome structure simultaneously on single chromosomes. We demonstrate that chromosomal translocations can alter transcription chromosome-wide, finding substantial differences in transcriptional frequency between...

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Detalles Bibliográficos
Autores principales: Levesque, Marshall J., Raj, Arjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4131260/
https://www.ncbi.nlm.nih.gov/pubmed/23416756
http://dx.doi.org/10.1038/nmeth.2372
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author Levesque, Marshall J.
Raj, Arjun
author_facet Levesque, Marshall J.
Raj, Arjun
author_sort Levesque, Marshall J.
collection PubMed
description Here we report iceFISH, a multiplex imaging method for measuring gene expression and chromosome structure simultaneously on single chromosomes. We demonstrate that chromosomal translocations can alter transcription chromosome-wide, finding substantial differences in transcriptional frequency between genes located on a translocated chromosome in comparison to the normal chromosome in the same cell. Examination of correlations between genes on a single chromosome revealed a cis chromosome-level transcriptional interaction spanning 14.3 megabases.
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spelling pubmed-41312602014-08-14 Single chromosome transcriptional profiling reveals chromosome-level regulation of gene expression Levesque, Marshall J. Raj, Arjun Nat Methods Article Here we report iceFISH, a multiplex imaging method for measuring gene expression and chromosome structure simultaneously on single chromosomes. We demonstrate that chromosomal translocations can alter transcription chromosome-wide, finding substantial differences in transcriptional frequency between genes located on a translocated chromosome in comparison to the normal chromosome in the same cell. Examination of correlations between genes on a single chromosome revealed a cis chromosome-level transcriptional interaction spanning 14.3 megabases. 2013-02-17 2013-03 /pmc/articles/PMC4131260/ /pubmed/23416756 http://dx.doi.org/10.1038/nmeth.2372 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Levesque, Marshall J.
Raj, Arjun
Single chromosome transcriptional profiling reveals chromosome-level regulation of gene expression
title Single chromosome transcriptional profiling reveals chromosome-level regulation of gene expression
title_full Single chromosome transcriptional profiling reveals chromosome-level regulation of gene expression
title_fullStr Single chromosome transcriptional profiling reveals chromosome-level regulation of gene expression
title_full_unstemmed Single chromosome transcriptional profiling reveals chromosome-level regulation of gene expression
title_short Single chromosome transcriptional profiling reveals chromosome-level regulation of gene expression
title_sort single chromosome transcriptional profiling reveals chromosome-level regulation of gene expression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4131260/
https://www.ncbi.nlm.nih.gov/pubmed/23416756
http://dx.doi.org/10.1038/nmeth.2372
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AT rajarjun singlechromosometranscriptionalprofilingrevealschromosomelevelregulationofgeneexpression

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