A reporter gene system for screening inhibitors of Wnt signaling pathway

Abnormal activation of canonical Wnt signaling has been associated with various types of cancer. Inhibitory reagents targeting the Wnt signaling have great potential to inhibit the growth of relevant tumors. Here we generated a cell-based screening strategy for identification of antagonists of the W...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Xing-Yao, Wang, Yuan-Yuan, Yuan, Chun-Mao, Hao, Xiao-Jiang, Li, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2013
Materias:
Regular Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4131617/
http://dx.doi.org/10.1007/s13659-012-0094-0
_version_ 1782330493928210432
author Li, Xing-Yao
Wang, Yuan-Yuan
Yuan, Chun-Mao
Hao, Xiao-Jiang
Li, Yan
author_facet Li, Xing-Yao
Wang, Yuan-Yuan
Yuan, Chun-Mao
Hao, Xiao-Jiang
Li, Yan
author_sort Li, Xing-Yao
collection PubMed
description Abnormal activation of canonical Wnt signaling has been associated with various types of cancer. Inhibitory reagents targeting the Wnt signaling have great potential to inhibit the growth of relevant tumors. Here we generated a cell-based screening strategy for identification of antagonists of the Wnt/β-catenin signaling pathway. Stable expression wnt3a was generated in HEK293 cell line, which harbors dual-luciferase reporters. The Wnt signaling in the stably transfected cell line was proved to be very sensitive to (−)-epigallocatechin-3-gallate (EGCG) and lithium chloride (LiCl) treatment, respectively. Natural compounds were screened and a couple of novel inhibitory modulators of the Wnt signaling pathway were obtained. [Image: see text]
format Online
Article
Text
id pubmed-4131617
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-41316172014-08-20 A reporter gene system for screening inhibitors of Wnt signaling pathway Li, Xing-Yao Wang, Yuan-Yuan Yuan, Chun-Mao Hao, Xiao-Jiang Li, Yan Nat Prod Bioprospect Regular Article Abnormal activation of canonical Wnt signaling has been associated with various types of cancer. Inhibitory reagents targeting the Wnt signaling have great potential to inhibit the growth of relevant tumors. Here we generated a cell-based screening strategy for identification of antagonists of the Wnt/β-catenin signaling pathway. Stable expression wnt3a was generated in HEK293 cell line, which harbors dual-luciferase reporters. The Wnt signaling in the stably transfected cell line was proved to be very sensitive to (−)-epigallocatechin-3-gallate (EGCG) and lithium chloride (LiCl) treatment, respectively. Natural compounds were screened and a couple of novel inhibitory modulators of the Wnt signaling pathway were obtained. [Image: see text] Springer Berlin Heidelberg 2013-01-09 /pmc/articles/PMC4131617/ http://dx.doi.org/10.1007/s13659-012-0094-0 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by/4.0/This article is published under license to BioMed Central Ltd. This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Regular Article
Li, Xing-Yao
Wang, Yuan-Yuan
Yuan, Chun-Mao
Hao, Xiao-Jiang
Li, Yan
A reporter gene system for screening inhibitors of Wnt signaling pathway
title A reporter gene system for screening inhibitors of Wnt signaling pathway
title_full A reporter gene system for screening inhibitors of Wnt signaling pathway
title_fullStr A reporter gene system for screening inhibitors of Wnt signaling pathway
title_full_unstemmed A reporter gene system for screening inhibitors of Wnt signaling pathway
title_short A reporter gene system for screening inhibitors of Wnt signaling pathway
title_sort reporter gene system for screening inhibitors of wnt signaling pathway
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4131617/
http://dx.doi.org/10.1007/s13659-012-0094-0
work_keys_str_mv AT lixingyao areportergenesystemforscreeninginhibitorsofwntsignalingpathway
AT wangyuanyuan areportergenesystemforscreeninginhibitorsofwntsignalingpathway
AT yuanchunmao areportergenesystemforscreeninginhibitorsofwntsignalingpathway
AT haoxiaojiang areportergenesystemforscreeninginhibitorsofwntsignalingpathway
AT liyan areportergenesystemforscreeninginhibitorsofwntsignalingpathway
AT lixingyao reportergenesystemforscreeninginhibitorsofwntsignalingpathway
AT wangyuanyuan reportergenesystemforscreeninginhibitorsofwntsignalingpathway
AT yuanchunmao reportergenesystemforscreeninginhibitorsofwntsignalingpathway
AT haoxiaojiang reportergenesystemforscreeninginhibitorsofwntsignalingpathway
AT liyan reportergenesystemforscreeninginhibitorsofwntsignalingpathway

Ejemplares similares