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Simultaneous Measurement of Smoothened Entry Into and Exit From the Primary Cilium

Ciliary accumulation of signaling proteins must result from a rate of ciliary entry that exceeds ciliary exit, but approaches for distinguishing ciliary entry vs. exit are lacking. Using a photoconvertible fluorescent protein tag, we establish an assay that allows a separate but simultaneous examina...

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Detalles Bibliográficos
Autores principales: Kim, Jynho, Hsia, Elaine Y. C., Kim, James, Sever, Navdar, Beachy, Philip A., Zheng, Xiaoyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4132089/
https://www.ncbi.nlm.nih.gov/pubmed/25119726
http://dx.doi.org/10.1371/journal.pone.0104070