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Simultaneous Measurement of Smoothened Entry Into and Exit From the Primary Cilium
Ciliary accumulation of signaling proteins must result from a rate of ciliary entry that exceeds ciliary exit, but approaches for distinguishing ciliary entry vs. exit are lacking. Using a photoconvertible fluorescent protein tag, we establish an assay that allows a separate but simultaneous examina...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4132089/ https://www.ncbi.nlm.nih.gov/pubmed/25119726 http://dx.doi.org/10.1371/journal.pone.0104070 |