Compartmentalized self-replication under fast PCR cycling conditions yields Taq DNA polymerase mutants with increased DNA-binding affinity and blood resistance

Faster-cycling PCR formulations, protocols, and instruments have been developed to address the need for increased throughput and shorter turn-around times for PCR-based assays. Although run times can be cut by up to 50%, shorter cycle times have been correlated with lower detection sensitivity and i...

Descripción completa

Detalles Bibliográficos
Autores principales: Arezi, Bahram, McKinney, Nancy, Hansen, Connie, Cayouette, Michelle, Fox, Jeffrey, Chen, Keith, Lapira, Jennifer, Hamilton, Sarah, Hogrefe, Holly
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4132270/
https://www.ncbi.nlm.nih.gov/pubmed/25177317
http://dx.doi.org/10.3389/fmicb.2014.00408

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4132270/
https://www.ncbi.nlm.nih.gov/pubmed/25177317
http://dx.doi.org/10.3389/fmicb.2014.00408

Ejemplares similares