Simultaneous non-contiguous deletions using large synthetic DNA and site-specific recombinases

Toward achieving rapid and large scale genome modification directly in a target organism, we have developed a new genome engineering strategy that uses a combination of bioinformatics aided design, large synthetic DNA and site-specific recombinases. Using Cre recombinase we swapped a target 126-kb s...

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Detalles Bibliográficos
Autores principales: Krishnakumar, Radha, Grose, Carissa, Haft, Daniel H., Zaveri, Jayshree, Alperovich, Nina, Gibson, Daniel G., Merryman, Chuck, Glass, John I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4132700/
https://www.ncbi.nlm.nih.gov/pubmed/24914053
http://dx.doi.org/10.1093/nar/gku509

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4132700/
https://www.ncbi.nlm.nih.gov/pubmed/24914053
http://dx.doi.org/10.1093/nar/gku509

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