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Comprehensive mutational scanning of a kinase in vivo reveals substrate-dependent fitness landscapes

Deep mutational scanning has emerged as a promising tool for mapping sequence–activity relationships in proteins, ribonucleic acid and deoxyribonucleic acid. In this approach, diverse variants of a sequence of interest are first ranked according to their activities in a relevant assay, and this rank...

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Detalles Bibliográficos
Autores principales: Melnikov, Alexandre, Rogov, Peter, Wang, Li, Gnirke, Andreas, Mikkelsen, Tarjei S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4132701/
https://www.ncbi.nlm.nih.gov/pubmed/24914046
http://dx.doi.org/10.1093/nar/gku511