Formation of circular polyribosomes on eukaryotic mRNA without cap-structure and poly(A)-tail: a cryo electron tomography study

The polyribosomes newly formed on recombinant GFP-encoding mRNAs in a wheat germ cell-free translation system were analyzed using cryo-electron tomography, with sub-tomogram averaging of polysomal ribosomes and reconstruction of 3D structures of individual polyribosomes. The achieved level of resolu...

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Detalles Bibliográficos
Autores principales: Afonina, Zhanna A., Myasnikov, Alexander G., Shirokov, Vladimir A., Klaholz, Bruno P., Spirin, Alexander S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Structural Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4132722/
https://www.ncbi.nlm.nih.gov/pubmed/25016525
http://dx.doi.org/10.1093/nar/gku599

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4132722/
https://www.ncbi.nlm.nih.gov/pubmed/25016525
http://dx.doi.org/10.1093/nar/gku599

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