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Expansion of the CRISPR-Cas9 genome targeting space through the use of H1 promoter-expressed guide–RNAs
The repurposed CRISPR-Cas9 system has recently emerged as a revolutionary genome-editing tool. Here we report a modification in the expression of the guide (gRNA) required for targeting that greatly expands the targetable genome. gRNA expression through the commonly used U6 promoter requires a guano...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4133144/ https://www.ncbi.nlm.nih.gov/pubmed/25105359 http://dx.doi.org/10.1038/ncomms5516 |