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Expansion of the CRISPR-Cas9 genome targeting space through the use of H1 promoter-expressed guide–RNAs

The repurposed CRISPR-Cas9 system has recently emerged as a revolutionary genome-editing tool. Here we report a modification in the expression of the guide (gRNA) required for targeting that greatly expands the targetable genome. gRNA expression through the commonly used U6 promoter requires a guano...

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Detalles Bibliográficos
Autores principales: Ranganathan, Vinod, Wahlin, Karl, Maruotti, Julien, Zack, Donald J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4133144/
https://www.ncbi.nlm.nih.gov/pubmed/25105359
http://dx.doi.org/10.1038/ncomms5516

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