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On non-detects in qPCR data

Motivation: Quantitative real-time PCR (qPCR) is one of the most widely used methods to measure gene expression. Despite extensive research in qPCR laboratory protocols, normalization and statistical analysis, little attention has been given to qPCR non-detects—those reactions failing to produce a m...

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Detalles Bibliográficos
Autores principales: McCall, Matthew N., McMurray, Helene R., Land, Hartmut, Almudevar, Anthony
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4133581/
https://www.ncbi.nlm.nih.gov/pubmed/24764462
http://dx.doi.org/10.1093/bioinformatics/btu239