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Supercritical fluid-mediated liposomes containing cyclosporin A for the treatment of dry eye syndrome in a rabbit model: comparative study with the conventional cyclosporin A emulsion
BACKGROUND: The objective of this study was to compare the efficacy of cyclosporin (CsA)-encapsulated liposomes with the commercially available CsA emulsion (Restasis(®)) for the treatment of dry eye syndrome in rabbits. METHODS: Liposomes containing CsA were prepared by the supercritical fluid (SCF...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4134020/ https://www.ncbi.nlm.nih.gov/pubmed/25143728 http://dx.doi.org/10.2147/IJN.S65601 |
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author | Karn, Pankaj Ranjan Kim, Hyun Do Kang, Han Sun, Bo Kyung Jin, Su-Eon Hwang, Sung-Joo |
author_facet | Karn, Pankaj Ranjan Kim, Hyun Do Kang, Han Sun, Bo Kyung Jin, Su-Eon Hwang, Sung-Joo |
author_sort | Karn, Pankaj Ranjan |
collection | PubMed |
description | BACKGROUND: The objective of this study was to compare the efficacy of cyclosporin (CsA)-encapsulated liposomes with the commercially available CsA emulsion (Restasis(®)) for the treatment of dry eye syndrome in rabbits. METHODS: Liposomes containing CsA were prepared by the supercritical fluid (SCF) method consisted of phosphatidylcholine from soybean (SCF-S100) and egg lecithins (SCF-EPCS). An in vitro permeation study was carried out using artificial cellulose membrane in Franz diffusion cells. Dry eye syndrome was induced in male albino rabbits and further subdivided into untreated, Restasis(®)-treated, EPCS, and S100-treated groups. Tear formation in the dry-eye-induced rabbits was evaluated using the Schirmer tear test. All formulations were also evaluated by ocular irritation tests using the Draize eye and winking methods with the determination of CsA concentration in rabbit tears. RESULTS: After the treatment, the Schirmer tear test value significantly improved in EPCS-treated (P=0.005) and S100-treated (P=0.018) groups compared to the Restasis(®)-treated group. The AUC(0–24 h) for rabbit’s tear film after the administration of SCF-S100 was 32.75±9.21 μg·h/mg which was significantly higher than that of 24.59±8.69 μg·h/mg reported with Restasis(®). Liposomal CsA formulations used in this study showed lower irritation in rabbit eyes compared with Restasis(®). CONCLUSION: These results demonstrate that the novel SCF-mediated liposomal CsA promises a significant improvement in overcoming the challenges associated with the treatment of dry eyes. |
format | Online Article Text |
id | pubmed-4134020 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-41340202014-08-20 Supercritical fluid-mediated liposomes containing cyclosporin A for the treatment of dry eye syndrome in a rabbit model: comparative study with the conventional cyclosporin A emulsion Karn, Pankaj Ranjan Kim, Hyun Do Kang, Han Sun, Bo Kyung Jin, Su-Eon Hwang, Sung-Joo Int J Nanomedicine Original Research BACKGROUND: The objective of this study was to compare the efficacy of cyclosporin (CsA)-encapsulated liposomes with the commercially available CsA emulsion (Restasis(®)) for the treatment of dry eye syndrome in rabbits. METHODS: Liposomes containing CsA were prepared by the supercritical fluid (SCF) method consisted of phosphatidylcholine from soybean (SCF-S100) and egg lecithins (SCF-EPCS). An in vitro permeation study was carried out using artificial cellulose membrane in Franz diffusion cells. Dry eye syndrome was induced in male albino rabbits and further subdivided into untreated, Restasis(®)-treated, EPCS, and S100-treated groups. Tear formation in the dry-eye-induced rabbits was evaluated using the Schirmer tear test. All formulations were also evaluated by ocular irritation tests using the Draize eye and winking methods with the determination of CsA concentration in rabbit tears. RESULTS: After the treatment, the Schirmer tear test value significantly improved in EPCS-treated (P=0.005) and S100-treated (P=0.018) groups compared to the Restasis(®)-treated group. The AUC(0–24 h) for rabbit’s tear film after the administration of SCF-S100 was 32.75±9.21 μg·h/mg which was significantly higher than that of 24.59±8.69 μg·h/mg reported with Restasis(®). Liposomal CsA formulations used in this study showed lower irritation in rabbit eyes compared with Restasis(®). CONCLUSION: These results demonstrate that the novel SCF-mediated liposomal CsA promises a significant improvement in overcoming the challenges associated with the treatment of dry eyes. Dove Medical Press 2014-08-08 /pmc/articles/PMC4134020/ /pubmed/25143728 http://dx.doi.org/10.2147/IJN.S65601 Text en © 2014 Karn et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Karn, Pankaj Ranjan Kim, Hyun Do Kang, Han Sun, Bo Kyung Jin, Su-Eon Hwang, Sung-Joo Supercritical fluid-mediated liposomes containing cyclosporin A for the treatment of dry eye syndrome in a rabbit model: comparative study with the conventional cyclosporin A emulsion |
title | Supercritical fluid-mediated liposomes containing cyclosporin A for the treatment of dry eye syndrome in a rabbit model: comparative study with the conventional cyclosporin A emulsion |
title_full | Supercritical fluid-mediated liposomes containing cyclosporin A for the treatment of dry eye syndrome in a rabbit model: comparative study with the conventional cyclosporin A emulsion |
title_fullStr | Supercritical fluid-mediated liposomes containing cyclosporin A for the treatment of dry eye syndrome in a rabbit model: comparative study with the conventional cyclosporin A emulsion |
title_full_unstemmed | Supercritical fluid-mediated liposomes containing cyclosporin A for the treatment of dry eye syndrome in a rabbit model: comparative study with the conventional cyclosporin A emulsion |
title_short | Supercritical fluid-mediated liposomes containing cyclosporin A for the treatment of dry eye syndrome in a rabbit model: comparative study with the conventional cyclosporin A emulsion |
title_sort | supercritical fluid-mediated liposomes containing cyclosporin a for the treatment of dry eye syndrome in a rabbit model: comparative study with the conventional cyclosporin a emulsion |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4134020/ https://www.ncbi.nlm.nih.gov/pubmed/25143728 http://dx.doi.org/10.2147/IJN.S65601 |
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