Multiplex Degenerate Primer Design for Targeted Whole Genome Amplification of Many Viral Genomes

Background. Targeted enrichment improves coverage of highly mutable viruses at low concentration in complex samples. Degenerate primers that anneal to conserved regions can facilitate amplification of divergent, low concentration variants, even when the strain present is unknown. Results. A tool for...

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Detalles Bibliográficos
Autores principales: Gardner, Shea N., Jaing, Crystal J., Elsheikh, Maher M., Peña, José, Hysom, David A., Borucki, Monica K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4137498/
https://www.ncbi.nlm.nih.gov/pubmed/25157264
http://dx.doi.org/10.1155/2014/101894

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4137498/
https://www.ncbi.nlm.nih.gov/pubmed/25157264
http://dx.doi.org/10.1155/2014/101894

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