SpeedyGenes: an improved gene synthesis method for the efficient production of error-corrected, synthetic protein libraries for directed evolution

The de novo synthesis of genes is becoming increasingly common in synthetic biology studies. However, the inherent error rate (introduced by errors incurred during oligonucleotide synthesis) limits its use in synthesising protein libraries to only short genes. Here we introduce SpeedyGenes, a PCR-ba...

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Detalles Bibliográficos
Autores principales: Currin, Andrew, Swainston, Neil, Day, Philip J., Kell, Douglas B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Original Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4140418/
https://www.ncbi.nlm.nih.gov/pubmed/25108914
http://dx.doi.org/10.1093/protein/gzu029

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4140418/
https://www.ncbi.nlm.nih.gov/pubmed/25108914
http://dx.doi.org/10.1093/protein/gzu029

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