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Selective Methyl Labeling of Eukaryotic Membrane Proteins Using Cell-Free Expression

[Image: see text] Structural characterization of membrane proteins and other large proteins with NMR relies increasingly on perdeuteration combined with incorporation of specifically protonated amino acid moieties, such as methyl groups of isoleucines, valines, or leucines. The resulting proton dilu...

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Detalles Bibliográficos
Autores principales: Linser, Rasmus, Gelev, Vladimir, Hagn, Franz, Arthanari, Haribabu, Hyberts, Sven G., Wagner, Gerhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4140455/
https://www.ncbi.nlm.nih.gov/pubmed/24937763
http://dx.doi.org/10.1021/ja504791j