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Selective Methyl Labeling of Eukaryotic Membrane Proteins Using Cell-Free Expression
[Image: see text] Structural characterization of membrane proteins and other large proteins with NMR relies increasingly on perdeuteration combined with incorporation of specifically protonated amino acid moieties, such as methyl groups of isoleucines, valines, or leucines. The resulting proton dilu...
Autores principales: | Linser, Rasmus, Gelev, Vladimir, Hagn, Franz, Arthanari, Haribabu, Hyberts, Sven G., Wagner, Gerhard |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2014
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4140455/ https://www.ncbi.nlm.nih.gov/pubmed/24937763 http://dx.doi.org/10.1021/ja504791j |
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