Development of a Prokaryotic Universal Primer for Simultaneous Analysis of Bacteria and Archaea Using Next-Generation Sequencing

For the analysis of microbial community structure based on 16S rDNA sequence diversity, sensitive and robust PCR amplification of 16S rDNA is a critical step. To obtain accurate microbial composition data, PCR amplification must be free of bias; however, amplifying all 16S rDNA species with equal ef...

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Detalles Bibliográficos
Autores principales: Takahashi, Shunsuke, Tomita, Junko, Nishioka, Kaori, Hisada, Takayoshi, Nishijima, Miyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4140814/
https://www.ncbi.nlm.nih.gov/pubmed/25144201
http://dx.doi.org/10.1371/journal.pone.0105592

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4140814/
https://www.ncbi.nlm.nih.gov/pubmed/25144201
http://dx.doi.org/10.1371/journal.pone.0105592

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