Conditional targeting of Ispd using paired Cas9 nickase and a single DNA template in mice

CRISPR/Cas9 technology is a highly promising genome editing tool in the mouse, potentially overcoming the costs and time required for more traditional gene targeting methods in embryonic stem (ES) cells. Recently, compared to the wildtype nuclease, paired Cas9 nickase (Cas9n) combined with single gu...

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Detalles Bibliográficos
Autores principales: Lee, Angus Yiu-fai, Lloyd, Kevin C. Kent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4141200/
https://www.ncbi.nlm.nih.gov/pubmed/25161872
http://dx.doi.org/10.1016/j.fob.2014.06.007

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4141200/
https://www.ncbi.nlm.nih.gov/pubmed/25161872
http://dx.doi.org/10.1016/j.fob.2014.06.007

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