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Optogenetic Recruitment of Dorsal Raphe Serotonergic Neurons Acutely Decreases Mechanosensory Responsivity in Behaving Mice

The inhibition of sensory responsivity is considered a core serotonin function, yet this hypothesis lacks direct support due to methodological obstacles. We adapted an optogenetic approach to induce acute, robust and specific firing of dorsal raphe serotonergic neurons. In vitro, the responsiveness...

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Autores principales: Dugué, Guillaume P., Lörincz, Magor L., Lottem, Eran, Audero, Enrica, Matias, Sara, Correia, Patricia A., Léna, Clément, Mainen, Zachary F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4141837/
https://www.ncbi.nlm.nih.gov/pubmed/25148042
http://dx.doi.org/10.1371/journal.pone.0105941
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author Dugué, Guillaume P.
Lörincz, Magor L.
Lottem, Eran
Audero, Enrica
Matias, Sara
Correia, Patricia A.
Léna, Clément
Mainen, Zachary F.
author_facet Dugué, Guillaume P.
Lörincz, Magor L.
Lottem, Eran
Audero, Enrica
Matias, Sara
Correia, Patricia A.
Léna, Clément
Mainen, Zachary F.
author_sort Dugué, Guillaume P.
collection PubMed
description The inhibition of sensory responsivity is considered a core serotonin function, yet this hypothesis lacks direct support due to methodological obstacles. We adapted an optogenetic approach to induce acute, robust and specific firing of dorsal raphe serotonergic neurons. In vitro, the responsiveness of individual dorsal raphe serotonergic neurons to trains of light pulses varied with frequency and intensity as well as between cells, and the photostimulation protocol was therefore adjusted to maximize their overall output rate. In vivo, the photoactivation of dorsal raphe serotonergic neurons gave rise to a prominent light-evoked field response that displayed some sensitivity to a 5-HT1A agonist, consistent with autoreceptor inhibition of raphe neurons. In behaving mice, the photostimulation of dorsal raphe serotonergic neurons produced a rapid and reversible decrease in the animals' responses to plantar stimulation, providing a new level of evidence that serotonin gates sensory-driven responses.
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spelling pubmed-41418372014-08-25 Optogenetic Recruitment of Dorsal Raphe Serotonergic Neurons Acutely Decreases Mechanosensory Responsivity in Behaving Mice Dugué, Guillaume P. Lörincz, Magor L. Lottem, Eran Audero, Enrica Matias, Sara Correia, Patricia A. Léna, Clément Mainen, Zachary F. PLoS One Research Article The inhibition of sensory responsivity is considered a core serotonin function, yet this hypothesis lacks direct support due to methodological obstacles. We adapted an optogenetic approach to induce acute, robust and specific firing of dorsal raphe serotonergic neurons. In vitro, the responsiveness of individual dorsal raphe serotonergic neurons to trains of light pulses varied with frequency and intensity as well as between cells, and the photostimulation protocol was therefore adjusted to maximize their overall output rate. In vivo, the photoactivation of dorsal raphe serotonergic neurons gave rise to a prominent light-evoked field response that displayed some sensitivity to a 5-HT1A agonist, consistent with autoreceptor inhibition of raphe neurons. In behaving mice, the photostimulation of dorsal raphe serotonergic neurons produced a rapid and reversible decrease in the animals' responses to plantar stimulation, providing a new level of evidence that serotonin gates sensory-driven responses. Public Library of Science 2014-08-22 /pmc/articles/PMC4141837/ /pubmed/25148042 http://dx.doi.org/10.1371/journal.pone.0105941 Text en © 2014 Dugué et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Dugué, Guillaume P.
Lörincz, Magor L.
Lottem, Eran
Audero, Enrica
Matias, Sara
Correia, Patricia A.
Léna, Clément
Mainen, Zachary F.
Optogenetic Recruitment of Dorsal Raphe Serotonergic Neurons Acutely Decreases Mechanosensory Responsivity in Behaving Mice
title Optogenetic Recruitment of Dorsal Raphe Serotonergic Neurons Acutely Decreases Mechanosensory Responsivity in Behaving Mice
title_full Optogenetic Recruitment of Dorsal Raphe Serotonergic Neurons Acutely Decreases Mechanosensory Responsivity in Behaving Mice
title_fullStr Optogenetic Recruitment of Dorsal Raphe Serotonergic Neurons Acutely Decreases Mechanosensory Responsivity in Behaving Mice
title_full_unstemmed Optogenetic Recruitment of Dorsal Raphe Serotonergic Neurons Acutely Decreases Mechanosensory Responsivity in Behaving Mice
title_short Optogenetic Recruitment of Dorsal Raphe Serotonergic Neurons Acutely Decreases Mechanosensory Responsivity in Behaving Mice
title_sort optogenetic recruitment of dorsal raphe serotonergic neurons acutely decreases mechanosensory responsivity in behaving mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4141837/
https://www.ncbi.nlm.nih.gov/pubmed/25148042
http://dx.doi.org/10.1371/journal.pone.0105941
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