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Elevated Levels of FMR1 mRNA in Granulosa Cells Are Associated with Low Ovarian Reserve in FMR1 Premutation Carriers
AIM: To assess the role of mRNA accumulation in granulosa cells as the cause of low ovarian response among FMR1 premutation carriers undergoing pre-implantation genetic diagnosis (PGD). DESIGN: Case control study in an academic IVF unit. Twenty-one consecutive FMR1 premutation carriers and 15 contro...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4143194/ https://www.ncbi.nlm.nih.gov/pubmed/25153074 http://dx.doi.org/10.1371/journal.pone.0105121 |
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author | Elizur, Shai E. Lebovitz, Oshrit Derech-Haim, Sanaz Dratviman-Storobinsky, Olga Feldman, Baruch Dor, Jehoshua Orvieto, Raoul Cohen, Yoram |
author_facet | Elizur, Shai E. Lebovitz, Oshrit Derech-Haim, Sanaz Dratviman-Storobinsky, Olga Feldman, Baruch Dor, Jehoshua Orvieto, Raoul Cohen, Yoram |
author_sort | Elizur, Shai E. |
collection | PubMed |
description | AIM: To assess the role of mRNA accumulation in granulosa cells as the cause of low ovarian response among FMR1 premutation carriers undergoing pre-implantation genetic diagnosis (PGD). DESIGN: Case control study in an academic IVF unit. Twenty-one consecutive FMR1 premutation carriers and 15 control women were included. After oocyte retrieval the granulosa cells mRNA levels of FMR1 was measured using RT-PCR. RESULTS: In FMR1 premutation carriers, there was a significant non-linear association between the number of CGG repeats and the number of retrieved oocytes (p<0.0001) and a trend to granulosa cells FMR1 mRNA levels (p = 0.07). The lowest number of retrieved oocytes and the highest level of mRNA were seen in women with mid-size CGG repeats (80–120). A significant negative linear correlation was observed between the granulosa cells FMR1 mRNA levels and the number of retrieved oocytes (R(2) linear = 0.231, P = 0.02). CONCLUSION: We suggest that there is a no-linear association between the number of CGG repeats and ovarian function, resulting from an increased granulosa cells FMR1 mRNA accumulation in FMR1 carriers in the mid-range (80–120 repeats). |
format | Online Article Text |
id | pubmed-4143194 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41431942014-08-27 Elevated Levels of FMR1 mRNA in Granulosa Cells Are Associated with Low Ovarian Reserve in FMR1 Premutation Carriers Elizur, Shai E. Lebovitz, Oshrit Derech-Haim, Sanaz Dratviman-Storobinsky, Olga Feldman, Baruch Dor, Jehoshua Orvieto, Raoul Cohen, Yoram PLoS One Research Article AIM: To assess the role of mRNA accumulation in granulosa cells as the cause of low ovarian response among FMR1 premutation carriers undergoing pre-implantation genetic diagnosis (PGD). DESIGN: Case control study in an academic IVF unit. Twenty-one consecutive FMR1 premutation carriers and 15 control women were included. After oocyte retrieval the granulosa cells mRNA levels of FMR1 was measured using RT-PCR. RESULTS: In FMR1 premutation carriers, there was a significant non-linear association between the number of CGG repeats and the number of retrieved oocytes (p<0.0001) and a trend to granulosa cells FMR1 mRNA levels (p = 0.07). The lowest number of retrieved oocytes and the highest level of mRNA were seen in women with mid-size CGG repeats (80–120). A significant negative linear correlation was observed between the granulosa cells FMR1 mRNA levels and the number of retrieved oocytes (R(2) linear = 0.231, P = 0.02). CONCLUSION: We suggest that there is a no-linear association between the number of CGG repeats and ovarian function, resulting from an increased granulosa cells FMR1 mRNA accumulation in FMR1 carriers in the mid-range (80–120 repeats). Public Library of Science 2014-08-25 /pmc/articles/PMC4143194/ /pubmed/25153074 http://dx.doi.org/10.1371/journal.pone.0105121 Text en © 2014 Elizur et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Elizur, Shai E. Lebovitz, Oshrit Derech-Haim, Sanaz Dratviman-Storobinsky, Olga Feldman, Baruch Dor, Jehoshua Orvieto, Raoul Cohen, Yoram Elevated Levels of FMR1 mRNA in Granulosa Cells Are Associated with Low Ovarian Reserve in FMR1 Premutation Carriers |
title | Elevated Levels of FMR1 mRNA in Granulosa Cells Are Associated with Low Ovarian Reserve in FMR1 Premutation Carriers |
title_full | Elevated Levels of FMR1 mRNA in Granulosa Cells Are Associated with Low Ovarian Reserve in FMR1 Premutation Carriers |
title_fullStr | Elevated Levels of FMR1 mRNA in Granulosa Cells Are Associated with Low Ovarian Reserve in FMR1 Premutation Carriers |
title_full_unstemmed | Elevated Levels of FMR1 mRNA in Granulosa Cells Are Associated with Low Ovarian Reserve in FMR1 Premutation Carriers |
title_short | Elevated Levels of FMR1 mRNA in Granulosa Cells Are Associated with Low Ovarian Reserve in FMR1 Premutation Carriers |
title_sort | elevated levels of fmr1 mrna in granulosa cells are associated with low ovarian reserve in fmr1 premutation carriers |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4143194/ https://www.ncbi.nlm.nih.gov/pubmed/25153074 http://dx.doi.org/10.1371/journal.pone.0105121 |
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