Simultaneous purification of nitrile hydratase and amidase of Alcaligenes sp. MTCC 10674

Alcaligenes sp. MTCC 10674 has a bienzymatic system for the hydrolysis of nitriles. The nitrile hydratase and amidase have been purified simultaneously to homogeneity using a combination of (NH)(4)SO(4) precipitation, ion exchange chromatography and gel permeation chromatography. Nitrile hydratase a...

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Detalles Bibliográficos
Autores principales: Bhatia, S. K., Mehta, P. K., Bhatia, R. K., Bhalla, T. C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2013
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4145624/
https://www.ncbi.nlm.nih.gov/pubmed/28324474
http://dx.doi.org/10.1007/s13205-013-0163-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4145624/
https://www.ncbi.nlm.nih.gov/pubmed/28324474
http://dx.doi.org/10.1007/s13205-013-0163-z

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