Chemokine receptor 4 gene silencing blocks neuroblastoma metastasis in vitro

This study investigated the effects of small interfering RNA (siRNA)-mediated silencing of chemokine receptor 4 (CXCR4) on the invasion capacity of human neuroblastoma cell line SH-SY5Y in vitro. Three siRNAs targeting CXCR4 were chemically synthesized and individually transfected into SH-SY5Y cells...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Xin, Zhu, Yongjie, Han, Lulu, Lu, Hongting, Hao, Xiwei, Dong, Qian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2014
Materias:
Technical Updates
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4146301/
https://www.ncbi.nlm.nih.gov/pubmed/25206760
http://dx.doi.org/10.4103/1673-5374.133172
_version_ 1782332329817014272
author Chen, Xin
Zhu, Yongjie
Han, Lulu
Lu, Hongting
Hao, Xiwei
Dong, Qian
author_facet Chen, Xin
Zhu, Yongjie
Han, Lulu
Lu, Hongting
Hao, Xiwei
Dong, Qian
author_sort Chen, Xin
collection PubMed
description This study investigated the effects of small interfering RNA (siRNA)-mediated silencing of chemokine receptor 4 (CXCR4) on the invasion capacity of human neuroblastoma cell line SH-SY5Y in vitro. Three siRNAs targeting CXCR4 were chemically synthesized and individually transfected into SH-SY5Y cells. Expression of CXCR4 mRNA and protein was significantly suppressed in transfected cells by all three sequence-specific siRNAs compared with control groups. Furthermore, the invasion capacity of SH-SY5Y cells was significantly decreased following transfection with CXCR4-specific siRNA compared with the control groups. These data demonstrate that down-regulation of CXCR4 can inhibit in vitro invasion of neuroblastoma.
format Online
Article
Text
id pubmed-4146301
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Medknow Publications & Media Pvt Ltd
record_format MEDLINE/PubMed
spelling pubmed-41463012014-09-09 Chemokine receptor 4 gene silencing blocks neuroblastoma metastasis in vitro Chen, Xin Zhu, Yongjie Han, Lulu Lu, Hongting Hao, Xiwei Dong, Qian Neural Regen Res Technical Updates This study investigated the effects of small interfering RNA (siRNA)-mediated silencing of chemokine receptor 4 (CXCR4) on the invasion capacity of human neuroblastoma cell line SH-SY5Y in vitro. Three siRNAs targeting CXCR4 were chemically synthesized and individually transfected into SH-SY5Y cells. Expression of CXCR4 mRNA and protein was significantly suppressed in transfected cells by all three sequence-specific siRNAs compared with control groups. Furthermore, the invasion capacity of SH-SY5Y cells was significantly decreased following transfection with CXCR4-specific siRNA compared with the control groups. These data demonstrate that down-regulation of CXCR4 can inhibit in vitro invasion of neuroblastoma. Medknow Publications & Media Pvt Ltd 2014-05-15 /pmc/articles/PMC4146301/ /pubmed/25206760 http://dx.doi.org/10.4103/1673-5374.133172 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Technical Updates
Chen, Xin
Zhu, Yongjie
Han, Lulu
Lu, Hongting
Hao, Xiwei
Dong, Qian
Chemokine receptor 4 gene silencing blocks neuroblastoma metastasis in vitro
title Chemokine receptor 4 gene silencing blocks neuroblastoma metastasis in vitro
title_full Chemokine receptor 4 gene silencing blocks neuroblastoma metastasis in vitro
title_fullStr Chemokine receptor 4 gene silencing blocks neuroblastoma metastasis in vitro
title_full_unstemmed Chemokine receptor 4 gene silencing blocks neuroblastoma metastasis in vitro
title_short Chemokine receptor 4 gene silencing blocks neuroblastoma metastasis in vitro
title_sort chemokine receptor 4 gene silencing blocks neuroblastoma metastasis in vitro
topic Technical Updates
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4146301/
https://www.ncbi.nlm.nih.gov/pubmed/25206760
http://dx.doi.org/10.4103/1673-5374.133172
work_keys_str_mv AT chenxin chemokinereceptor4genesilencingblocksneuroblastomametastasisinvitro
AT zhuyongjie chemokinereceptor4genesilencingblocksneuroblastomametastasisinvitro
AT hanlulu chemokinereceptor4genesilencingblocksneuroblastomametastasisinvitro
AT luhongting chemokinereceptor4genesilencingblocksneuroblastomametastasisinvitro
AT haoxiwei chemokinereceptor4genesilencingblocksneuroblastomametastasisinvitro
AT dongqian chemokinereceptor4genesilencingblocksneuroblastomametastasisinvitro

Ejemplares similares