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No protein intake compensation for insufficient indispensable amino acid intake with a low-protein diet for 12 days

BACKGROUND: Protein quality evaluation aims to determine the capacity of food sources and diets to meet protein and indispensable amino acid (IAA) requirements. This study determined whether nitrogen balance was affected and whether dietary IAA were adequately obtained from the ad libitum consumptio...

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Autores principales: Martens, Eveline A, Tan, Sze-Yen, Mattes, Richard D, Westerterp-Plantenga, Margriet S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4147096/
https://www.ncbi.nlm.nih.gov/pubmed/25183991
http://dx.doi.org/10.1186/1743-7075-11-38
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author Martens, Eveline A
Tan, Sze-Yen
Mattes, Richard D
Westerterp-Plantenga, Margriet S
author_facet Martens, Eveline A
Tan, Sze-Yen
Mattes, Richard D
Westerterp-Plantenga, Margriet S
author_sort Martens, Eveline A
collection PubMed
description BACKGROUND: Protein quality evaluation aims to determine the capacity of food sources and diets to meet protein and indispensable amino acid (IAA) requirements. This study determined whether nitrogen balance was affected and whether dietary IAA were adequately obtained from the ad libitum consumption of diets at three levels of protein from different primary sources for 12 days. METHODS: Two 12-day randomized crossover design trials were conducted in healthy subjects [n = 70/67 (M/F); age: 19-70 y; BMI: 18.2-38.7 kg/m(2)]. The relative dietary protein content was lower than [5% of energy (En%)], similar to (15En%), and higher than (30En%) customary diets. These diets had a limited variety of protein sources, containing wheat protein as a single protein source (5En%-protein diet) or 5En% from wheat protein with 10En% (15En%-protein diets) or 25En% (30En%-protein diets) added from whey with α-lactalbumin, soy or beef protein. RESULTS: There was a dose-dependent increase in nitrogen excretion with increasing dietary protein content, irrespective of the protein sources (P = 0.001). Nitrogen balance was maintained on the 5En%-protein diet, and was positive on the 15En%- and 30En%-protein diets (P < 0.001) over 12 days. Protein intake from the 5En%-protein diet did not reach the amount necessary to meet the calculated minimal IAA requirements, but IAA were sufficiently obtained from the 15En%- and 30En%-protein diets. In the 15En%- and 30En%-protein conditions, a higher protein intake from the soy-containing diets than from the whey with α-lactalbumin or beef containing diets was needed to meet the minimal IAA requirements. CONCLUSION: Protein intake did not compensate for an insufficient indispensable amino acid intake with a low-protein diet for 12 days. TRIAL REGISTRATION: These trials were registered at clinicaltrials.gov as NCT01320189 and NCT01646749.
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spelling pubmed-41470962014-09-02 No protein intake compensation for insufficient indispensable amino acid intake with a low-protein diet for 12 days Martens, Eveline A Tan, Sze-Yen Mattes, Richard D Westerterp-Plantenga, Margriet S Nutr Metab (Lond) Research BACKGROUND: Protein quality evaluation aims to determine the capacity of food sources and diets to meet protein and indispensable amino acid (IAA) requirements. This study determined whether nitrogen balance was affected and whether dietary IAA were adequately obtained from the ad libitum consumption of diets at three levels of protein from different primary sources for 12 days. METHODS: Two 12-day randomized crossover design trials were conducted in healthy subjects [n = 70/67 (M/F); age: 19-70 y; BMI: 18.2-38.7 kg/m(2)]. The relative dietary protein content was lower than [5% of energy (En%)], similar to (15En%), and higher than (30En%) customary diets. These diets had a limited variety of protein sources, containing wheat protein as a single protein source (5En%-protein diet) or 5En% from wheat protein with 10En% (15En%-protein diets) or 25En% (30En%-protein diets) added from whey with α-lactalbumin, soy or beef protein. RESULTS: There was a dose-dependent increase in nitrogen excretion with increasing dietary protein content, irrespective of the protein sources (P = 0.001). Nitrogen balance was maintained on the 5En%-protein diet, and was positive on the 15En%- and 30En%-protein diets (P < 0.001) over 12 days. Protein intake from the 5En%-protein diet did not reach the amount necessary to meet the calculated minimal IAA requirements, but IAA were sufficiently obtained from the 15En%- and 30En%-protein diets. In the 15En%- and 30En%-protein conditions, a higher protein intake from the soy-containing diets than from the whey with α-lactalbumin or beef containing diets was needed to meet the minimal IAA requirements. CONCLUSION: Protein intake did not compensate for an insufficient indispensable amino acid intake with a low-protein diet for 12 days. TRIAL REGISTRATION: These trials were registered at clinicaltrials.gov as NCT01320189 and NCT01646749. BioMed Central 2014-08-20 /pmc/articles/PMC4147096/ /pubmed/25183991 http://dx.doi.org/10.1186/1743-7075-11-38 Text en Copyright © 2014 Martens et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Martens, Eveline A
Tan, Sze-Yen
Mattes, Richard D
Westerterp-Plantenga, Margriet S
No protein intake compensation for insufficient indispensable amino acid intake with a low-protein diet for 12 days
title No protein intake compensation for insufficient indispensable amino acid intake with a low-protein diet for 12 days
title_full No protein intake compensation for insufficient indispensable amino acid intake with a low-protein diet for 12 days
title_fullStr No protein intake compensation for insufficient indispensable amino acid intake with a low-protein diet for 12 days
title_full_unstemmed No protein intake compensation for insufficient indispensable amino acid intake with a low-protein diet for 12 days
title_short No protein intake compensation for insufficient indispensable amino acid intake with a low-protein diet for 12 days
title_sort no protein intake compensation for insufficient indispensable amino acid intake with a low-protein diet for 12 days
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4147096/
https://www.ncbi.nlm.nih.gov/pubmed/25183991
http://dx.doi.org/10.1186/1743-7075-11-38
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