Expression, purification and reconstitution of the 4-hydroxybenzoate transporter PcaK from Acinetobacter sp. ADP1()

The aromatic acid:H(+) symporter family of integral membrane proteins play an important role in the microbial metabolism of aromatic compounds. Here, we show that the 4-hydroxybenzoate transporter from Acinetobacter sp. ADP1, PcaK, can be successfully overexpressed in Escherichia coli and purified b...

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Detalles Bibliográficos
Autores principales: Pernstich, Christian, Senior, Laura, MacInnes, Katherine A., Forsaith, Marc, Curnow, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4148202/
https://www.ncbi.nlm.nih.gov/pubmed/24907408
http://dx.doi.org/10.1016/j.pep.2014.05.011

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4148202/
https://www.ncbi.nlm.nih.gov/pubmed/24907408
http://dx.doi.org/10.1016/j.pep.2014.05.011

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