Isolation of rare recombinants without using selectable markers for one-step seamless BAC mutagenesis

Current laboratory methods to isolate rare (1:10,000 to 1:100,000) bacterial artificial chromosome (BAC) recombinants require selectable markers. Seamless BAC mutagenesis needs two steps: isolation of rare recombinants using selectable markers, followed by marker removal through counterselection. He...

Descripción completa

Detalles Bibliográficos
Autores principales: Lyozin, George T., Bressloff, Paul C., Kumar, Amit, Kosaka, Yasuhiro, Demarest, Bradley L., Yost, H. Joseph, Kuehn, Michael R., Brunelli, Luca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4149595/
https://www.ncbi.nlm.nih.gov/pubmed/25028895
http://dx.doi.org/10.1038/nmeth.3030

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4149595/
https://www.ncbi.nlm.nih.gov/pubmed/25028895
http://dx.doi.org/10.1038/nmeth.3030

Ejemplares similares