Fluorescent TEM-1 β-lactamase with wild-type activity as a rapid drug sensor for in vitro drug screening

We report the development of a novel fluorescent drug sensor from the bacterial drug target TEM-1 β-lactamase through the combined strategy of Val(216)→Cys(216) mutation and fluorophore labelling for in vitro drug screening. The Val(216) residue in TEM-1 is replaced with a cysteine residue, and the...

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Detalles Bibliográficos
Autores principales: Cheong, Wing-Lam, Tsang, Ming-San, So, Pui-Kin, Chung, Wai-Hong, Leung, Yun-Chung, Chan, Pak-Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2014
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4155835/
https://www.ncbi.nlm.nih.gov/pubmed/25074398
http://dx.doi.org/10.1042/BSR20140057

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4155835/
https://www.ncbi.nlm.nih.gov/pubmed/25074398
http://dx.doi.org/10.1042/BSR20140057

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