Application of Locked Nucleic Acid (LNA) Oligonucleotide–PCR Clamping Technique to Selectively PCR Amplify the SSU rRNA Genes of Bacteria in Investigating the Plant-Associated Community Structures

The simultaneous extraction of plant organelle (mitochondria and plastid) genes during the DNA extraction step is a major limitation in investigating the community structures of bacteria associated with plants because organelle SSU rRNA genes are easily amplified by PCR using primer sets that are sp...

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Detalles Bibliográficos
Autores principales: Ikenaga, Makoto, Sakai, Masao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Microbial Ecology (JSME)/The Japanese Society of Soil Microbiology (JSSM) 2014
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4159040/
https://www.ncbi.nlm.nih.gov/pubmed/25030190
http://dx.doi.org/10.1264/jsme2.ME14061

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4159040/
https://www.ncbi.nlm.nih.gov/pubmed/25030190
http://dx.doi.org/10.1264/jsme2.ME14061

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