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Application of Locked Nucleic Acid (LNA) Oligonucleotide–PCR Clamping Technique to Selectively PCR Amplify the SSU rRNA Genes of Bacteria in Investigating the Plant-Associated Community Structures

The simultaneous extraction of plant organelle (mitochondria and plastid) genes during the DNA extraction step is a major limitation in investigating the community structures of bacteria associated with plants because organelle SSU rRNA genes are easily amplified by PCR using primer sets that are sp...

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Detalles Bibliográficos
Autores principales:	Ikenaga, Makoto, Sakai, Masao
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Japanese Society of Microbial Ecology (JSME)/The Japanese Society of Soil Microbiology (JSSM) 2014
Materias:	Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4159040/ https://www.ncbi.nlm.nih.gov/pubmed/25030190 http://dx.doi.org/10.1264/jsme2.ME14061

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