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Regulation of Apelin and Its Receptor Expression in Adipose Tissues of Obesity Rats with Hypertension and Cultured 3T3-L1 Adipocytes
The apelin/APJ system has been implicated in obesity-related hypertension. We investigated the mechanism responsible for the pathogenesis of obesity-related hypertension with a special focus on the crosstalk between AngII/its type 1 receptor (AT1R) signaling and apelin/APJ expression. Sprague-Dawley...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japanese Association for Laboratory Animal Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4160987/ https://www.ncbi.nlm.nih.gov/pubmed/24770651 http://dx.doi.org/10.1538/expanim.63.257 |
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author | Wu, Hongxian Cheng, Xian Wu Hao, Changning Zhang, Zhi Yao, Huali Murohara, Toyoaki Dai, Qiuyan |
author_facet | Wu, Hongxian Cheng, Xian Wu Hao, Changning Zhang, Zhi Yao, Huali Murohara, Toyoaki Dai, Qiuyan |
author_sort | Wu, Hongxian |
collection | PubMed |
description | The apelin/APJ system has been implicated in obesity-related hypertension. We investigated the mechanism responsible for the pathogenesis of obesity-related hypertension with a special focus on the crosstalk between AngII/its type 1 receptor (AT1R) signaling and apelin/APJ expression. Sprague-Dawley rats fed a high-fat (obesity-related hypertension, OH) or normal-fat diet (NF) for 15 weeks were randomly assigned to one of two groups and administered vehicle or perindopril for 4 weeks. Compared to the NF rats, the OH rats showed lower levels of plasma apelin and apelin/APJ mRNAs of perirenal adipose tissues, and these changes were restored by perindopril. Administration of the AT1R antagonist olmesartan resulted in the restoration of the reduction of apelin and APJ expressions induced by AngII for 48 h in 3T3-L1 adipocytes. Among several inhibitors for extracellular signal-regulated kinases 1/2 (ERK1/2) PD98059, p38 mitogen-activated protein kinase (p38MAPK) SB203580 and phosphatidylinositol 3-kinase (PI3K) LY294002, the latter showed an additive effect on AngII-mediated inhibitory effects. In addition, the levels of p-Akt, p-ERK and p38MAPK proteins were decreased by long-term treatment with AngII (120 min), and these changes were restored by Olmesartan. Apelin/APJ appears to be impaired in obesity-related hypertension. The AngII inhibition-mediated beneficial effects are likely attributable, at least in part, to restoration of p38/ERK-dependent apelin/APJ expression in diet-induced obesity-related hypertension. |
format | Online Article Text |
id | pubmed-4160987 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Japanese Association for Laboratory Animal Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41609872014-10-21 Regulation of Apelin and Its Receptor Expression in Adipose Tissues of Obesity Rats with Hypertension and Cultured 3T3-L1 Adipocytes Wu, Hongxian Cheng, Xian Wu Hao, Changning Zhang, Zhi Yao, Huali Murohara, Toyoaki Dai, Qiuyan Exp Anim Original The apelin/APJ system has been implicated in obesity-related hypertension. We investigated the mechanism responsible for the pathogenesis of obesity-related hypertension with a special focus on the crosstalk between AngII/its type 1 receptor (AT1R) signaling and apelin/APJ expression. Sprague-Dawley rats fed a high-fat (obesity-related hypertension, OH) or normal-fat diet (NF) for 15 weeks were randomly assigned to one of two groups and administered vehicle or perindopril for 4 weeks. Compared to the NF rats, the OH rats showed lower levels of plasma apelin and apelin/APJ mRNAs of perirenal adipose tissues, and these changes were restored by perindopril. Administration of the AT1R antagonist olmesartan resulted in the restoration of the reduction of apelin and APJ expressions induced by AngII for 48 h in 3T3-L1 adipocytes. Among several inhibitors for extracellular signal-regulated kinases 1/2 (ERK1/2) PD98059, p38 mitogen-activated protein kinase (p38MAPK) SB203580 and phosphatidylinositol 3-kinase (PI3K) LY294002, the latter showed an additive effect on AngII-mediated inhibitory effects. In addition, the levels of p-Akt, p-ERK and p38MAPK proteins were decreased by long-term treatment with AngII (120 min), and these changes were restored by Olmesartan. Apelin/APJ appears to be impaired in obesity-related hypertension. The AngII inhibition-mediated beneficial effects are likely attributable, at least in part, to restoration of p38/ERK-dependent apelin/APJ expression in diet-induced obesity-related hypertension. Japanese Association for Laboratory Animal Science 2014-04-26 2014 /pmc/articles/PMC4160987/ /pubmed/24770651 http://dx.doi.org/10.1538/expanim.63.257 Text en ©2014 Japanese Association for Laboratory Animal Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. |
spellingShingle | Original Wu, Hongxian Cheng, Xian Wu Hao, Changning Zhang, Zhi Yao, Huali Murohara, Toyoaki Dai, Qiuyan Regulation of Apelin and Its Receptor Expression in Adipose Tissues of Obesity Rats with Hypertension and Cultured 3T3-L1 Adipocytes |
title | Regulation of Apelin and Its Receptor Expression in Adipose Tissues of
Obesity Rats with Hypertension and Cultured 3T3-L1 Adipocytes |
title_full | Regulation of Apelin and Its Receptor Expression in Adipose Tissues of
Obesity Rats with Hypertension and Cultured 3T3-L1 Adipocytes |
title_fullStr | Regulation of Apelin and Its Receptor Expression in Adipose Tissues of
Obesity Rats with Hypertension and Cultured 3T3-L1 Adipocytes |
title_full_unstemmed | Regulation of Apelin and Its Receptor Expression in Adipose Tissues of
Obesity Rats with Hypertension and Cultured 3T3-L1 Adipocytes |
title_short | Regulation of Apelin and Its Receptor Expression in Adipose Tissues of
Obesity Rats with Hypertension and Cultured 3T3-L1 Adipocytes |
title_sort | regulation of apelin and its receptor expression in adipose tissues of
obesity rats with hypertension and cultured 3t3-l1 adipocytes |
topic | Original |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4160987/ https://www.ncbi.nlm.nih.gov/pubmed/24770651 http://dx.doi.org/10.1538/expanim.63.257 |
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