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High-Throughput Bead-Based Identification of Structure-Switching Aptamer Beacons
We describe a new platform to identify structure-switching DNA beacon aptamers, which detect small molecules in a specific manner. By clonally amplifying a DNA library designed to fluoresce in response to binding events onto microbeads, aptamer beacons can be selected by stringent fluorescence-assis...
Autores principales: | Trevino, Simon G, Levy, Matthew |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
WILEY-VCH Verlag
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161366/ https://www.ncbi.nlm.nih.gov/pubmed/25056925 http://dx.doi.org/10.1002/cbic.201402037 |
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