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Validation of four reference genes for quantitative mRNA expression studies in a rat model of inflammatory injury
BACKGROUND: Real-time quantitative PCR (qPCR) is a technique frequently used to measure changes in mRNA expression. To ensure validity of experimental findings, it is important to normalize the qPCR data to reference genes that are stable and unaffected by the experimental treatment to correct for v...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161874/ https://www.ncbi.nlm.nih.gov/pubmed/25187167 http://dx.doi.org/10.1186/1744-8069-10-55 |