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Validation of four reference genes for quantitative mRNA expression studies in a rat model of inflammatory injury

BACKGROUND: Real-time quantitative PCR (qPCR) is a technique frequently used to measure changes in mRNA expression. To ensure validity of experimental findings, it is important to normalize the qPCR data to reference genes that are stable and unaffected by the experimental treatment to correct for v...

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Detalles Bibliográficos
Autores principales: Walder, Roxanne Y, Wattiez, Anne-Sophie, White, Stephanie R, Marquez de Prado, Blanca, Hamity, Marta V, Hammond, Donna L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161874/
https://www.ncbi.nlm.nih.gov/pubmed/25187167
http://dx.doi.org/10.1186/1744-8069-10-55