Selection of chromosomal DNA libraries using a multiplex CRISPR system

The directed evolution of biomolecules to improve or change their activity is central to many engineering and synthetic biology efforts. However, selecting improved variants from gene libraries in living cells requires plasmid expression systems that suffer from variable copy number effects, or the...

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Detalles Bibliográficos
Autores principales: Ryan, Owen W, Skerker, Jeffrey M, Maurer, Matthew J, Li, Xin, Tsai, Jordan C, Poddar, Snigdha, Lee, Michael E, DeLoache, Will, Dueber, John E, Arkin, Adam P, Cate, Jamie HD
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2014
Materias:
Biophysics and Structural Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161972/
https://www.ncbi.nlm.nih.gov/pubmed/25139909
http://dx.doi.org/10.7554/eLife.03703

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161972/
https://www.ncbi.nlm.nih.gov/pubmed/25139909
http://dx.doi.org/10.7554/eLife.03703

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