Refolding and purification of recombinant L-asparaginase from inclusion bodies of E. coli into active tetrameric protein

A tetrameric protein of therapeutic importance, Escherichia coli L-asparaginase-II was expressed in Escherichia coli as inclusion bodies (IBs). Asparaginase IBs were solubilized using low concentration of urea and refolded into active tetrameric protein using pulsatile dilution method. Refolded aspa...

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Detalles Bibliográficos
Autores principales: Upadhyay, Arun K., Singh, Anupam, Mukherjee, K. J., Panda, Amulya K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4164012/
https://www.ncbi.nlm.nih.gov/pubmed/25309524
http://dx.doi.org/10.3389/fmicb.2014.00486

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4164012/
https://www.ncbi.nlm.nih.gov/pubmed/25309524
http://dx.doi.org/10.3389/fmicb.2014.00486

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