Cargando…

Refolding and purification of recombinant L-asparaginase from inclusion bodies of E. coli into active tetrameric protein

A tetrameric protein of therapeutic importance, Escherichia coli L-asparaginase-II was expressed in Escherichia coli as inclusion bodies (IBs). Asparaginase IBs were solubilized using low concentration of urea and refolded into active tetrameric protein using pulsatile dilution method. Refolded aspa...

Descripción completa

Detalles Bibliográficos
Autores principales:	Upadhyay, Arun K., Singh, Anupam, Mukherjee, K. J., Panda, Amulya K.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2014
Materias:	Microbiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4164012/ https://www.ncbi.nlm.nih.gov/pubmed/25309524 http://dx.doi.org/10.3389/fmicb.2014.00486

Ejemplares similares

Structure-Function Relationship of Inclusion Bodies of a Multimeric Protein
por: Singh, Anupam, et al.
Publicado: (2020)

Molecular Attributes Associated With Refolding of Inclusion Body Proteins Using the Freeze–Thaw Method
por: Singhvi, Priyank, et al.
Publicado: (2021)

Kinetics of Inclusion Body Formation and Its Correlation with the Characteristics of Protein Aggregates in Escherichia coli
por: Upadhyay, Arun K., et al.
Publicado: (2012)

Protein recovery from inclusion bodies of Escherichia coli using mild solubilization process
por: Singh, Anupam, et al.
Publicado: (2015)

Inclusion Body Expression and Refolding of Recombinant Bone Morphogenetic Protein-2
por: Nasrabadi, Davood, et al.
Publicado: (2018)

Recovery of bioactive protein from bacterial inclusion bodies using trifluoroethanol as solubilization agent
por: Upadhyay, Vaibhav, et al.
Publicado: (2016)

Refolding Techniques for Recovering Biologically Active Recombinant Proteins from Inclusion Bodies
por: Yamaguchi, Hiroshi, et al.
Publicado: (2014)

Thermoresponsive Gigaporous Microspheres Facilitate the Efficient Refolding of Recombinant Nitrilase Inclusion Bodies
por: Qu, Jian-Bo, et al.
Publicado: (2020)

Enhancement of Solubility, Purification, and Inclusion Body Refolding of Active Human Mitochondrial Aldehyde Dehydrogenase 2
por: Zhao, Tingting, et al.
Publicado: (2021)

Production, purification and characterization of l-asparaginase from streptomyces gulbargensis
por: Amena, S., et al.
Publicado: (2010)

Chemical Assistance in Refolding of Bacterial Inclusion Bodies
por: Alibolandi, Mona, et al.
Publicado: (2011)

Retraction of “Thermoresponsive Gigaporous Microspheres Facilitate the Efficient Refolding of Recombinant Nitrilase Inclusion Bodies”
por: Qu, Jian-Bo, et al.
Publicado: (2021)

Refolding and characterization of two G protein-coupled receptors purified from E. coli inclusion bodies
por: Heim, Bastian, et al.
Publicado: (2021)

Simplified Large-Scale Refolding, Purification, and Characterization of Recombinant Human Granulocyte-Colony Stimulating Factor in Escherichia coli
por: Kim, Chang Kyu, et al.
Publicado: (2013)

A Simplified Method for the Efficient Refolding and Purification of Recombinant Human GM-CSF
por: Thomson, Christy A., et al.
Publicado: (2012)

Purification and Refolding of Overexpressed Human Basic Fibroblast Growth Factor in Escherichia coli
por: Alibolandi, Mona, et al.
Publicado: (2011)

Effect of buffer additives on solubilization and refolding of reteplase inclusion bodies
por: Esmaili, Iman, et al.
Publicado: (2018)

MB109 as bioactive human bone morphogenetic protein-9 refolded and purified from E. coli inclusion bodies
por: Kuo, Mario Meng-Chiang, et al.
Publicado: (2014)

Generalized enzymatic mechanism of catalysis by tetrameric l-asparaginases from mesophilic bacteria
por: Strzelczyk, Pawel, et al.
Publicado: (2020)

A Simplified Process for Purification and Refolding of Recombinant Human Interferon-α2b
por: Hezarjaribi, Nima, et al.
Publicado: (2022)

Expression in Escherichia coli, purification, refolding and antifungal activity of an osmotin from Solanum nigrum
por: Campos, Magnólia de A, et al.
Publicado: (2008)

Strategies for the recovery of active proteins through refolding of bacterial inclusion body proteins
por: Vallejo, Luis Felipe, et al.
Publicado: (2004)

Cloning, expression, purification and characterisation of Erwinia carotovora L-asparaginase in Escherichia coli
por: Pourhossein, Meraj, et al.
Publicado: (2014)

Expression of SARS-CoV-2 surface glycoprotein fragment 319–640 in E. coli, and its refolding and purification
por: Fitzgerald, Gabriel A., et al.
Publicado: (2021)

Production of soluble truncated spike protein of porcine epidemic diarrhea virus from inclusion bodies of Escherichia coli through refolding
por: Piao, Da-Chuan, et al.
Publicado: (2016)

Allergic Reactions to E. coli Asparaginase are Associated with Decreased Asparaginase Activity in an Indonesian Pediatric Population with ALL
por: Sari, Nur Melani, et al.
Publicado: (2023)

Efficient in vitro refolding and functional characterization of recombinant human liver carboxylesterase (CES1) expressed in E. coli
por: Boonyuen, Usa, et al.
Publicado: (2015)

Refolding of bioactive human epidermal growth factor from E. coli BL21(DE3) inclusion bodies & evaluations on its in vitro & in vivo bioactivity
por: Maksum, Iman Permana, et al.
Publicado: (2022)

The REFOLD database: a tool for the optimization of protein expression and refolding
por: Chow, Michelle K. M., et al.
Publicado: (2006)

Purification and Characterization of Asparaginase from Phaseolus vulgaris Seeds
por: Mohamed, Saleh A., et al.
Publicado: (2015)

Efficient recovery of recombinant CRM197 expressed as inclusion bodies in E.coli
por: Park, Ah-Reum, et al.
Publicado: (2018)

Quality comparison of a state-of-the-art preparation of a recombinant L-asparaginase derived from Escherichia coli with an alternative asparaginase product
por: Schnuchel, Arndt, et al.
Publicado: (2023)

Expression, Purification, Refolding, and Characterization of a Neverland Protein From Caenorhabditis elegans
por: Mao, Shuhong, et al.
Publicado: (2020)

Affinity Based Nano-Magnetic Particles for Purification of Recombinant Proteins in Form of Inclusion Body
por: Seyedinkhorasani, Masoud, et al.
Publicado: (2020)

Tetramerization of SAMHD1 Is Required for Biological Activity and Inhibition of HIV Infection
por: Yan, Junpeng, et al.
Publicado: (2013)

Effect of pressure on refolding of recombinant pentameric cholera toxin B
por: Rodrigues, D., et al.
Publicado: (2014)

The effect of thioredoxin and prochymosin coexpression on the refolding of recombinant alpaca chymosin
por: Belenkaya, S.V., et al.
Publicado: (2023)

Is monkeypox a threat to another pandemic?
por: Panda, Kingshuk, et al.
Publicado: (2022)

Prokaryotic expression, refolding, and purification of fragment 450–650 of the spike protein of SARS-coronavirus
por: Zhao, Jin-Cun, et al.
Publicado: (2005)

Evaluation of Recombinant Human Growth Hormone Secretion in E. coli using the L-asparaginase II Signal Peptide
por: Zamani, Mozhdeh, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_v2muijcdh30rtku0qjep3qlobl