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The Characterisation of an Alkali-Stable Maltogenic Amylase from Bacillus lehensis G1 and Improved Malto-Oligosaccharide Production by Hydrolysis Suppression

A maltogenic amylase (MAG1) from alkaliphilic Bacillus lehensis G1 was cloned, expressed in Escherichia coli, purified and characterised for its hydrolysis and transglycosylation properties. The enzyme exhibited high stability at pH values from 7.0 to 10.0. The hydrolysis of β-cyclodextrin (β-CD) pr...

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Detalles Bibliográficos
Autores principales: Abdul Manas, Nor Hasmaliana, Pachelles, Samson, Mahadi, Nor Muhammad, Illias, Rosli Md.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4164441/
https://www.ncbi.nlm.nih.gov/pubmed/25221964
http://dx.doi.org/10.1371/journal.pone.0106481