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A Rapid and Simple Method for DNA Engineering Using Cycled Ligation Assembly

DNA assembly techniques have developed rapidly, enabling efficient construction of complex constructs that would be prohibitively difficult using traditional restriction-digest based methods. Most of the recent methods for assembling multiple DNA fragments in vitro suffer from high costs, complex se...

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Detalles Bibliográficos
Autores principales: Roth, Theodore L., Milenkovic, Ljiljana, Scott, Matthew P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4167330/
https://www.ncbi.nlm.nih.gov/pubmed/25226397
http://dx.doi.org/10.1371/journal.pone.0107329