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A Rapid and Simple Method for DNA Engineering Using Cycled Ligation Assembly
DNA assembly techniques have developed rapidly, enabling efficient construction of complex constructs that would be prohibitively difficult using traditional restriction-digest based methods. Most of the recent methods for assembling multiple DNA fragments in vitro suffer from high costs, complex se...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2014
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4167330/ https://www.ncbi.nlm.nih.gov/pubmed/25226397 http://dx.doi.org/10.1371/journal.pone.0107329 |