Fluorescent-increase kinetics of different fluorescent reporters used for qPCR depend on monitoring chemistry, targeted sequence, type of DNA input and PCR efficiency

The analysis of quantitative PCR data usually does not take into account the fact that the increase in fluorescence depends on the monitoring chemistry, the input of ds-DNA or ss-cDNA, and the directionality of the targeting of probes or primers. The monitoring chemistries currently available can be...

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Detalles Bibliográficos
Autores principales: Ruijter, Jan M., Lorenz, Peter, Tuomi, Jari M., Hecker, Michael, van den Hoff, Maurice J. B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2014
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4167442/
https://www.ncbi.nlm.nih.gov/pubmed/25253910
http://dx.doi.org/10.1007/s00604-013-1155-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4167442/
https://www.ncbi.nlm.nih.gov/pubmed/25253910
http://dx.doi.org/10.1007/s00604-013-1155-8

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