Introducing Micrometer-Sized Artificial Objects into Live Cells: A Method for Cell–Giant Unilamellar Vesicle Electrofusion

Here, we report a method for introducing large objects of up to a micrometer in diameter into cultured mammalian cells by electrofusion of giant unilamellar vesicles. We prepared GUVs containing various artificial objects using a water-in-oil (w/o) emulsion centrifugation method. GUVs and dispersed...

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Detalles Bibliográficos
Autores principales: Saito, Akira C., Ogura, Toshihiko, Fujiwara, Kei, Murata, Satoshi, Nomura, Shin-ichiro M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4167692/
https://www.ncbi.nlm.nih.gov/pubmed/25229561
http://dx.doi.org/10.1371/journal.pone.0106853

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4167692/
https://www.ncbi.nlm.nih.gov/pubmed/25229561
http://dx.doi.org/10.1371/journal.pone.0106853

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