Optimization of Extraction of Circulating RNAs from Plasma – Enabling Small RNA Sequencing

There are several protocols and kits for the extraction of circulating RNAs from plasma with a following quantification of specific genes via RT-qPCR. Due to the marginal amount of cell-free RNA in plasma samples, the total RNA yield is insufficient to perform Next-Generation Sequencing (NGS), the s...

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Detalles Bibliográficos
Autores principales: Spornraft, Melanie, Kirchner, Benedikt, Haase, Bettina, Benes, Vladimir, Pfaffl, Michael W., Riedmaier, Irmgard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4167851/
https://www.ncbi.nlm.nih.gov/pubmed/25229651
http://dx.doi.org/10.1371/journal.pone.0107259

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4167851/
https://www.ncbi.nlm.nih.gov/pubmed/25229651
http://dx.doi.org/10.1371/journal.pone.0107259

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