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Optimization of Extraction of Circulating RNAs from Plasma – Enabling Small RNA Sequencing

There are several protocols and kits for the extraction of circulating RNAs from plasma with a following quantification of specific genes via RT-qPCR. Due to the marginal amount of cell-free RNA in plasma samples, the total RNA yield is insufficient to perform Next-Generation Sequencing (NGS), the s...

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Detalles Bibliográficos
Autores principales:	Spornraft, Melanie, Kirchner, Benedikt, Haase, Bettina, Benes, Vladimir, Pfaffl, Michael W., Riedmaier, Irmgard
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2014
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4167851/ https://www.ncbi.nlm.nih.gov/pubmed/25229651 http://dx.doi.org/10.1371/journal.pone.0107259

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