A Genetic Assay for Transcription Errors Reveals Multilayer Control of RNA Polymerase II Fidelity

We developed a highly sensitive assay to detect transcription errors in vivo. The assay is based on suppression of a missense mutation in the active site tyrosine in the Cre recombinase. Because Cre acts as tetramer, background from translation errors are negligible. Functional Cre resulting from ra...

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Detalles Bibliográficos
Autores principales: Irvin, Jordan D., Kireeva, Maria L., Gotte, Deanna R., Shafer, Brenda K., Huang, Ingold, Kashlev, Mikhail, Strathern, Jeffrey N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4168980/
https://www.ncbi.nlm.nih.gov/pubmed/25232834
http://dx.doi.org/10.1371/journal.pgen.1004532

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4168980/
https://www.ncbi.nlm.nih.gov/pubmed/25232834
http://dx.doi.org/10.1371/journal.pgen.1004532

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