Cargando…

A Genetic Assay for Transcription Errors Reveals Multilayer Control of RNA Polymerase II Fidelity

We developed a highly sensitive assay to detect transcription errors in vivo. The assay is based on suppression of a missense mutation in the active site tyrosine in the Cre recombinase. Because Cre acts as tetramer, background from translation errors are negligible. Functional Cre resulting from ra...

Descripción completa

Detalles Bibliográficos
Autores principales:	Irvin, Jordan D., Kireeva, Maria L., Gotte, Deanna R., Shafer, Brenda K., Huang, Ingold, Kashlev, Mikhail, Strathern, Jeffrey N.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2014
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4168980/ https://www.ncbi.nlm.nih.gov/pubmed/25232834 http://dx.doi.org/10.1371/journal.pgen.1004532

Ejemplares similares

A Cre Transcription Fidelity Reporter Identifies GreA as a Major RNA Proofreading Factor in Escherichia coli
por: Bubunenko, Mikhail G., et al.
Publicado: (2017)

Complete dissection of transcription elongation reveals slow translocation of RNA polymerase II in a linear ratchet mechanism
por: Dangkulwanich, Manchuta, et al.
Publicado: (2013)

RNA–DNA and DNA–DNA base-pairing at the upstream edge of the transcription bubble regulate translocation of RNA polymerase and transcription rate
por: KIreeva, Maria, et al.
Publicado: (2018)

The fidelity of transcription in human cells
por: Chung, Claire, et al.
Publicado: (2023)

Visualizing translocation dynamics and nascent transcript errors in paused RNA polymerases in vivo
por: Imashimizu, Masahiko, et al.
Publicado: (2015)

Erratum to: Visualizing translocation dynamics and nascent transcript errors in paused RNA polymerases in vivo
por: Imashimizu, Masahiko, et al.
Publicado: (2015)

Direct assessment of transcription fidelity by high-resolution RNA sequencing
por: Imashimizu, Masahiko, et al.
Publicado: (2013)

Synergistic action of RNA polymerases in overcoming the nucleosomal barrier
por: Jin, Jing, et al.
Publicado: (2010)

Transcription elongation: Heterogeneous tracking of RNA polymerase and its biological implications
por: Imashimizu, Masahiko, et al.
Publicado: (2014)

Molecular dynamics and mutational analysis of the catalytic and translocation cycle of RNA polymerase
por: Kireeva, Maria L, et al.
Publicado: (2012)

Sensitivity of Mitochondrial Transcription and Resistance of RNA Polymerase II Dependent Nuclear Transcription to Antiviral Ribonucleosides
por: Arnold, Jamie J., et al.
Publicado: (2012)

The high fidelity and unique error signature of human DNA polymerase ε
por: Korona, Dagmara A., et al.
Publicado: (2011)

Bacteriophage λ N protein inhibits transcription slippage by Escherichia coli RNA polymerase
por: Parks, Adam R., et al.
Publicado: (2014)

Elevated Mutation Rate during Meiosis in Saccharomyces cerevisiae
por: Rattray, Alison, et al.
Publicado: (2015)

The architecture of RNA polymerase fidelity
por: Kaplan, Craig D
Publicado: (2010)

Temperature effect on polymerase fidelity
por: Xue, Yuan, et al.
Publicado: (2021)

Plasmid-based lacZα assay for DNA polymerase fidelity: application to archaeal family-B DNA polymerase
por: Jozwiakowski, Stanislaw K., et al.
Publicado: (2009)

Impact of Polymerase Fidelity on Background Error Rates in Next-Generation Sequencing with Unique Molecular Identifiers/Barcodes
por: Filges, Stefan, et al.
Publicado: (2019)

Five checkpoints maintaining the fidelity of transcription by RNA polymerases in structural and energetic details
por: Wang, Beibei, et al.
Publicado: (2015)

Compatibility and Fidelity of Mirror-Image Thymidine in Transcription Events by T7 RNA Polymerase
por: Liu, Qingju, et al.
Publicado: (2020)

Effects of Friedreich's ataxia (GAA)(n)·(TTC)(n) repeats on RNA synthesis and stability
por: Krasilnikova, Maria M., et al.
Publicado: (2007)

The elongation rate of RNA polymerase determines the fate of transcribed nucleosomes
por: Bintu, Lacramioara, et al.
Publicado: (2011)

A plasmid-based lacZα gene assay for DNA polymerase fidelity measurement
por: Keith, Brian J., et al.
Publicado: (2013)

In Silico Design and Enzymatic Synthesis of Functional RNA Nanoparticles
por: Afonin, Kirill A., et al.
Publicado: (2014)

Loop II of DNA polymerase beta is important for polymerization activity and fidelity
por: Lin, George C., et al.
Publicado: (2007)

High fidelity TNA synthesis by Therminator polymerase
por: Ichida, Justin K., et al.
Publicado: (2005)

Translocation and fidelity of Escherichia coli RNA polymerase
por: Nedialkov, Yuri A., et al.
Publicado: (2013)

A Critical Residue Selectively Recruits Nucleotides for T7 RNA Polymerase Transcription Fidelity Control
por: Duan, Baogen, et al.
Publicado: (2014)

Loop 1 modulates the fidelity of DNA polymerase λ
por: Bebenek, Katarzyna, et al.
Publicado: (2010)

RB69 DNA Polymerase Structure, Kinetics, and Fidelity
por: Xia, Shuangluo, et al.
Publicado: (2014)

Norovirus Polymerase Fidelity Contributes to Viral Transmission In Vivo
por: Arias, Armando, et al.
Publicado: (2016)

A trailing ribosome speeds up RNA polymerase at the expense of transcript fidelity via force and allostery
por: Wee, Liang Meng, et al.
Publicado: (2023)

MutS regulates access of the error-prone DNA polymerase Pol IV to replication sites: a novel mechanism for maintaining replication fidelity
por: Margara, Lucía M., et al.
Publicado: (2016)

Kinetics of DNA strand transfer between polymerase and proofreading exonuclease active sites regulates error correction during high-fidelity replication
por: Dangerfield, Tyler L., et al.
Publicado: (2022)

Low-fidelity DNA synthesis by human DNA polymerase theta
por: Arana, Mercedes E., et al.
Publicado: (2008)

Biochemical characterization of the fidelity of poliovirus RNA-dependent RNA polymerase
por: Freistadt, Marion S, et al.
Publicado: (2007)

Generation and characterization of influenza A viruses with altered polymerase fidelity
por: Cheung, Peter PH, et al.
Publicado: (2014)

Base modifications affecting RNA polymerase and reverse transcriptase fidelity
por: Potapov, Vladimir, et al.
Publicado: (2018)

Oxidative damage diminishes mitochondrial DNA polymerase replication fidelity
por: Anderson, Andrew P, et al.
Publicado: (2020)

Fidelity of DNA polymerases in the detection of intraindividual variation of mitochondrial DNA
por: Ricardo, Paulo Cseri, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_oatf944vsnfk90f3n5mv10k4d6