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Phytochemical Profiles and Antioxidant Activities in Six Species of Ramie Leaves

Increased consumption of vegetables or plant food has been associated with decreased risk of developing major chronic diseases, such as cancers, diabetes, cardiovascular diseases, and age-related functional decline. Ramie leaves are rich in phenolics and flavonoids, which have been suggested for hum...

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Autores principales: Chen, Yongsheng, Wang, Gaoyan, Wang, Hong, Cheng, Chaohua, Zang, Gonggu, Guo, Xinbo, Liu, Rui Hai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4171523/
https://www.ncbi.nlm.nih.gov/pubmed/25243741
http://dx.doi.org/10.1371/journal.pone.0108140
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author Chen, Yongsheng
Wang, Gaoyan
Wang, Hong
Cheng, Chaohua
Zang, Gonggu
Guo, Xinbo
Liu, Rui Hai
author_facet Chen, Yongsheng
Wang, Gaoyan
Wang, Hong
Cheng, Chaohua
Zang, Gonggu
Guo, Xinbo
Liu, Rui Hai
author_sort Chen, Yongsheng
collection PubMed
description Increased consumption of vegetables or plant food has been associated with decreased risk of developing major chronic diseases, such as cancers, diabetes, cardiovascular diseases, and age-related functional decline. Ramie leaves are rich in phenolics and flavonoids, which have been suggested for human health benefits. Phenolic contents, flavonoid contents, phenolic compounds, and anti-cancer properties in six species of ramie leaves were analyzed by Folin-reagent method, sodium borohydride/chloranil-based assay (SBC), HPLC method and antiproliferation, cytoxicity, respectively. Antioxidant activities were measured through peroxyl radical scavenging capacity (PSC) method, oxygen radical absorbance capacity (ORAC) method, and cellular antioxidant activity (CAA). Research indicated that Boehmeria penduliflora contained the highest total phenolic content (2313.7±27.28 mg GAE/100 g FW), and flavonoid content (1682.4±27.70 mg CAE/100 g FW). Boehmeria tricuspis showed the highest PSC value (9574.8±117.63 µM vit. C equiv./100 g FW), while Boehmeria penduliflora indicated the highest ORAC value (330.44±16.88 µmol Trolox equiv./g FW). The antioxidant activities were correlated with phenolic contents and flavonoid contents. Boehmeria tricuspis had the highest antiproliferative capacity with the lowest EC(50) (4.11±0.19 mg/mL). The results for the analyzed ramie for CAA were significantly different from each other (p<0.05), Boehmeria tricuspis had the highest CAA value (133.63±7.10 µmol QE/100 g). Benzoic acid, 4-coumaric acid, caffeic acid, and ferulic acid were the dominant phenolic ingredients in the ramie leaves according to HPLC analysis. Our research is the first report to study the phytochemical profiles and antioxidant activities in different species of ramie leaves for their health benefit.
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spelling pubmed-41715232014-09-25 Phytochemical Profiles and Antioxidant Activities in Six Species of Ramie Leaves Chen, Yongsheng Wang, Gaoyan Wang, Hong Cheng, Chaohua Zang, Gonggu Guo, Xinbo Liu, Rui Hai PLoS One Research Article Increased consumption of vegetables or plant food has been associated with decreased risk of developing major chronic diseases, such as cancers, diabetes, cardiovascular diseases, and age-related functional decline. Ramie leaves are rich in phenolics and flavonoids, which have been suggested for human health benefits. Phenolic contents, flavonoid contents, phenolic compounds, and anti-cancer properties in six species of ramie leaves were analyzed by Folin-reagent method, sodium borohydride/chloranil-based assay (SBC), HPLC method and antiproliferation, cytoxicity, respectively. Antioxidant activities were measured through peroxyl radical scavenging capacity (PSC) method, oxygen radical absorbance capacity (ORAC) method, and cellular antioxidant activity (CAA). Research indicated that Boehmeria penduliflora contained the highest total phenolic content (2313.7±27.28 mg GAE/100 g FW), and flavonoid content (1682.4±27.70 mg CAE/100 g FW). Boehmeria tricuspis showed the highest PSC value (9574.8±117.63 µM vit. C equiv./100 g FW), while Boehmeria penduliflora indicated the highest ORAC value (330.44±16.88 µmol Trolox equiv./g FW). The antioxidant activities were correlated with phenolic contents and flavonoid contents. Boehmeria tricuspis had the highest antiproliferative capacity with the lowest EC(50) (4.11±0.19 mg/mL). The results for the analyzed ramie for CAA were significantly different from each other (p<0.05), Boehmeria tricuspis had the highest CAA value (133.63±7.10 µmol QE/100 g). Benzoic acid, 4-coumaric acid, caffeic acid, and ferulic acid were the dominant phenolic ingredients in the ramie leaves according to HPLC analysis. Our research is the first report to study the phytochemical profiles and antioxidant activities in different species of ramie leaves for their health benefit. Public Library of Science 2014-09-22 /pmc/articles/PMC4171523/ /pubmed/25243741 http://dx.doi.org/10.1371/journal.pone.0108140 Text en © 2014 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Chen, Yongsheng
Wang, Gaoyan
Wang, Hong
Cheng, Chaohua
Zang, Gonggu
Guo, Xinbo
Liu, Rui Hai
Phytochemical Profiles and Antioxidant Activities in Six Species of Ramie Leaves
title Phytochemical Profiles and Antioxidant Activities in Six Species of Ramie Leaves
title_full Phytochemical Profiles and Antioxidant Activities in Six Species of Ramie Leaves
title_fullStr Phytochemical Profiles and Antioxidant Activities in Six Species of Ramie Leaves
title_full_unstemmed Phytochemical Profiles and Antioxidant Activities in Six Species of Ramie Leaves
title_short Phytochemical Profiles and Antioxidant Activities in Six Species of Ramie Leaves
title_sort phytochemical profiles and antioxidant activities in six species of ramie leaves
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4171523/
https://www.ncbi.nlm.nih.gov/pubmed/25243741
http://dx.doi.org/10.1371/journal.pone.0108140
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