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HIGH SENSITIVE PCR METHOD FOR DETECTION OF PATHOGENIC Leptospira spp. IN PARAFFIN-EMBEDDED TISSUES

This study describes the development and application of a new PCR assay for the specific detection of pathogenic leptospires and its comparison with a previously reported PCR protocol. New primers were designed for PCR optimization and evaluation in artificially-infected paraffin-embedded tissues. P...

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Detalles Bibliográficos
Autores principales: Noda, Angel Alberto, Rodríguez, Islay, Rodríguez, Yaindrys, Govín, Anamays, Fernández, Carmen, Obregón, Ana Margarita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto de Medicina Tropical 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4172112/
https://www.ncbi.nlm.nih.gov/pubmed/25229221
http://dx.doi.org/10.1590/S0036-46652014000500007