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Setting Up a Simple Light Sheet Microscope for In Toto Imaging of C. elegans Development

Fast and low phototoxic imaging techniques are pre-requisite to study the development of organisms in toto. Light sheet based microscopy reduces photo-bleaching and phototoxic effects compared to confocal microscopy, while providing 3D images with subcellular resolution. Here we present the setup of...

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Detalles Bibliográficos
Autores principales: Chardès, Claire, Mélénec, Pauline, Bertrand, Vincent, Lenne, Pierre-François
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4172305/
https://www.ncbi.nlm.nih.gov/pubmed/24836407
http://dx.doi.org/10.3791/51342