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Simultaneous high-resolution detection of multiple transcripts combined with localization of proteins in whole-mount embryos

BACKGROUND: Whole-mount in situ hybridization (WISH) is a fundamental tool for studying the spatio-temporal expression pattern of RNA molecules in intact embryos and tissues. The available methodologies for detecting mRNAs in embryos rely on enzymatic activities and chemical reactions that generate...

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Detalles Bibliográficos
Autores principales: Gross-Thebing, Theresa, Paksa, Azadeh, Raz, Erez
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4172952/
https://www.ncbi.nlm.nih.gov/pubmed/25124741
http://dx.doi.org/10.1186/s12915-014-0055-7