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Simultaneous high-resolution detection of multiple transcripts combined with localization of proteins in whole-mount embryos
BACKGROUND: Whole-mount in situ hybridization (WISH) is a fundamental tool for studying the spatio-temporal expression pattern of RNA molecules in intact embryos and tissues. The available methodologies for detecting mRNAs in embryos rely on enzymatic activities and chemical reactions that generate...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4172952/ https://www.ncbi.nlm.nih.gov/pubmed/25124741 http://dx.doi.org/10.1186/s12915-014-0055-7 |