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Simultaneous high-resolution detection of multiple transcripts combined with localization of proteins in whole-mount embryos

BACKGROUND: Whole-mount in situ hybridization (WISH) is a fundamental tool for studying the spatio-temporal expression pattern of RNA molecules in intact embryos and tissues. The available methodologies for detecting mRNAs in embryos rely on enzymatic activities and chemical reactions that generate...

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Detalles Bibliográficos
Autores principales:	Gross-Thebing, Theresa, Paksa, Azadeh, Raz, Erez
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2014
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4172952/ https://www.ncbi.nlm.nih.gov/pubmed/25124741 http://dx.doi.org/10.1186/s12915-014-0055-7

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